PCTAIRE Antibody

The K184R point mutation in PCTAIRE-1 results in redistribution of the ER-Golgi SNARE protein membrin to a more dispersed localization , and a reduction in the intensity and juxtanuclear clustering of COPII-coated ERES marked with Sec24Dp. As well as the variant PCTAIRE motif, the CDK16 fold shows differences to other CDKs in the conformation of the CDK/MAPK insert . The CDK/MAPK insert forms an additional protein–protein interaction surface in the CDK family.
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In the validation phase, RNA was extracted from 250 μl plasma. Briefly, a 250-μl plasma aliquot was mixed with one milliliter TRIzol LS reagent. After 5 min of incubation, 266 μl of chloroform was added to the mixture. Then, the mixture was centrifuged at × g for 15 min at 4°C. An equal volume of isopropanol and 1 μl GlycoBlue was added to the solution to precipitate RNA.
The serum which is used should match the species of the secondary antibody. The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Antibody Performance Guarantee. A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads. Pre-clear enough lysate for test samples and isotype controls. The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein.

Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. RNA overexpressed in cancer tissues is not necessarily expressed at a high level in the peripheral blood. This is because the tumor mass accounts only for a small portion of the body, and both the tumor tissue and other normal tissues contribute RNAs to the circulation. Most RNAs elevated in tumor tissues are thus masked by background RNAs from normal tissues. Therefore, the RNA candidates overexpressed in tumor tissues are not necessarily good markers in the circulation.
Immunoprecipitated proteins were then detected by immunoblotting using anti-Sec23Ap or anti-PCTAIRE-1/3 antibodies . The crystal structures of monomeric CDK16 represent the first structures from the PCTAIRE family of CDK kinases . The kinase domain exhibits the classical bilobal architecture intermixed with short insertions that help to define the CDK family fold. The complex with indirubin E804 displays some disorder in the kinase N-lobe, consistent with its requirement for a cyclin partner.

This protein, designated cyclindependent kinase 2 , also binds to cyclins and its kinase activity  is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE. This protein designated cyclin dependent kinase 2 p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PCTAIRE 1. Antibodies are purified by protein A and peptide affinity chromatography. For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight. These tissue lysates can be used in applications such as SDS-PAGE and Western blotting. Whole cell lysates can be used as positive controls for applications such as ELISAs, immunoprecipitation and Western blotting. Obtaining high-quality whole cell lysates can be tedious as well as time consuming.
The third member of this family, PCTK3, has been the least studied. Although exogenously expressed PCTK1 and PCTK2 phosphorylate myelin basic protein and histone H1 in vitro, PCTK3 kinase activity has not been detected. To characterize PCTK3 activity and elucidate its regulatory mechanism, it is necessary to identify activators for PCTK3. PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport.

The guaranteed shelf life from date of receipt for antibodies and conjugates is listed on the product information sheet. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. The Web Site may contain links to other websites on the internet. The presence of links from the Web Site to any third party website does not mean that we approve of, endorse or recommend that website.
The urea moiety's nitrogens also form two hydrogen bonds with Glu211 from the αC ‘PCTAIRE’ motif. A further hydrogen bond is formed between the backbone amide of Asp304 and the urea carbonyl . The central phenyl ring is sandwiched between the gatekeeper residue Phe240 and the flipped-out Phe305 , providing strong π–π packing interactions. The t-butyl group occupies the hydrophobic pocket vacated by the inverted Phe305. Finally, the quinoline moiety extends below Glu211 and the αC helix . CDK16 and ABL show many side chain differences in this region that could be useful for the future modification of rebastinib to more selectively target CDK16.

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Abcepta offers a comprehensive portfolio of whole cell lysates for research use. Browse our catalog to find the right whole cell lysate for your experiment. The majority of Abcepta antibodies are produced using peptide immunogens. These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications. Identification of PCTK1 as an elevated circulating mRNA in NSCLC patients. The PCTK1 mRNA levels in the plasma were  determined by reverse transcription quantitative PCR and normalized to that of the β2-microglobulin transcript.
In addition, we found that PCTK3 contains four putative PKA phosphorylation sites (Arg-Arg-X-Ser/Thr) and that PCTK3 phosphorylation by PKA results in an increase in its catalytic activity even in the absence of cyclin A2. These findings provide some insights into the functions of PCTK3. Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase.